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Distribution and daily variations of PACAP in the chicken brain.

机译:PACAP在鸡脑中的分布和日变化。

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Levels of PACAP38 were measured in different areas of the chicken brain under various lighting conditions by radioimmunoassay (RIA). Selected groups of animals were maintained under light for 14 h alternating with 10 h of darkness (LD), reversed lighting conditions (DL) and constant light (LL) or constant dark (DD). Daily variations of PACAP levels were observed in the brainstem, diencephalon, telencephalon and retina. In the brainstem and diencephalon, levels of PACAP increased during subjective nighttime, except in the DL group where levels were elevated between 15-21 h. In the telencephalon, the lowest level of PACAP was measured between 12-21 h except in the DL group where two peaks occurred at 18 and 03 h. In the retina, all 4 groups showed a similar level and pattern, with lowest levels during midday hours. No daily variation was observed in the pineal gland. According to the present observations, it is suggested that PACAP levels differ in several areas of the chicken brain under various lighting conditions and photic stimuli do not appear to be the main regulators of the circadian variations of PACAP.
机译:通过放射免疫测定(RIA)在各种光照条件下测量了鸡脑不同区域的PACAP38水平。选定的动物群在光照下保持14小时,同时保持10小时的黑暗(LD),反向照明条件(DL)和恒定的光照(LL)或恒定的黑暗(DD)。在脑干,中脑,远脑和视网膜中观察到PACAP水平的每日变化。在脑干和间脑,夜间主观夜间PACAP水平升高,但DL组在15-21 h之间升高。在末梢脑中,PACAP的最低水平是在12-21小时之间测得的,而DL组则在18和03小时出现两个峰值。在视网膜中,所有四个组的水平和模式相似,中午时分最低。在松果体中未观察到每日变化。根据目前的观察结果,建议在各种光照条件下,鸡脑几个区域的PACAP水平不同,并且光刺激似乎不是PACAP昼夜节律变化的主要调节因子。

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