Effects of N 2-alkylguanine, O 6-alkylguanine, and abasic lesions on DNA binding and bypass synthesis by the euryarchaeal B-family DNA polymerase vent (exo -)

摘要

Archaeal and eukaryotic B-family DNA polymerases (pols) mainly replicate chromosomal DNA but stall at lesions, which are often bypassed with Y-family pols. In this study, a B-family pol Vent (exo -) from the euryarchaeon Thermococcus litoralis was studied with three types of DNA lesions-N 2-alkylG, O 6-alkylG, and an abasic (AP) site-in comparison with a model Y-family pol Dpo4 from Sulfolobus solfataricus, to better understand the effects of various DNA modifications on binding, bypass efficiency, and fidelity of pols. Vent (exo -) readily bypassed N 2-methyl(Me)G and O 6-MeG, but was strongly blocked at O 6-benzyl(Bz)G and N 2-BzG, whereas Dpo4 efficiently bypassed N 2-MeG and N 2-BzG and partially bypassed O 6-MeG and O 6-BzG. Vent (exo -) bypassed an AP site to an extent greater than Dpo4, corresponding with steady-state kinetic data. Vent (exo -) showed ～110-, 180-, and 300-fold decreases in catalytic efficiency (k cat/K m) for nucleotide insertion opposite an AP site, N 2-MeG, and O 6-MeG but ～1800- and 5000-fold decreases opposite O 6-BzG and N 2-BzG, respectively, as compared to G, whereas Dpo4 showed little or only ～13-fold decreases opposite N 2-MeG and N 2-BzG but ～260-370-fold decreases opposite O 6-MeG, O 6-BzG, and the AP site. Vent (exo -) preferentially misinserted G opposite N 2-MeG, T opposite O 6-MeG, and A opposite an AP site and N 2-BzG, while Dpo4 favored correct C insertion opposite those lesions. Vent (exo -) and Dpo4 both bound modified DNAs with affinities similar to unmodified DNA. Our results indicate that Vent (exo -) is as or more efficient as Dpo4 in synthesis opposite O 6-MeG and AP lesions, whereas Dpo4 is much or more efficient opposite (only) N 2-alkylGs than Vent (exo -), irrespective of DNA-binding affinity. Our data also suggest that Vent (exo -) accepts nonbulky DNA lesions (e.g., N 2- or O 6-MeG and an AP site) as manageable substrates despite causing error-prone synthesis, whereas Dpo4 strongly favors minor-groove N 2-alkylG lesions over major-groove or noninstructive lesions.