Probing the Dynamics of Protein–Protein Interactions at Neuronal Contacts by Optical Imaging

摘要

In this review, we give an overview of the various optical-based methods allowing characterization of the assembly and turnover of macromolecular complexes present at neuronal contacts. We first briefly explain the biological paradigm and relevant molecular components. Then, we describe state of the art microscopy techniques that allow a dynamic visualization of specific elements at such contact sites. We focus sequentially on optical tweezers, FRAP, single-molecule and particle tracking, FCCS, and FRET/FLIM. Further, we outline in each case the mathematical analysis required to extract the physicochemical parameters that define protein–protein interactions. For illustration, we present recent data obtained in our laboratory as well as work from other groups, referring to a few specific examples. We conclude by comparing the pros and cons of these various approaches.