Covalent immobilization of albumin on micron-sized magnetic poly(methyl methacrylate-divinylbenzene-glycidyl methacrylate) microspheres prepared by modified suspension polymerization

摘要

Micron-sized magnetic poly(methyl methacrylate-divinylbenzene-glycidyl methacrylate) micro-spheres were prepared by a modified suspension polymerization in the presence of oleic acid-coated magnetite nanoparticles. The magnetic microspheres were functionalized by reacting the epoxy groups with ammonia solution to provide amino groups. After activated with glutaraldehyde (GA), bovine serum albumin was covalently immobilized on these magnetic microspheres. The influence of initial protein concentration, pH and ionic strength of the protein solution on covalent immobilization was studied. Scanning electron micrographs showed that the magnetic micro-spheres had an average size of 6.4μm and relative narrow size distribution. Magnetic measurement revealed the magnetic microspheres were superparamagetic with saturation magnetization of 7.32emu/g. The successful animation of the magnetic microspheres was confirmed by Fourier transform infrared spectroscopy (FT-IR).