The CLEAR supports were originally developed for solid-phase synthesis.In this study,two wide-pore versions of the resins were prepared by radical suspension polymerization using toluene as the porogen.Trimethylolpropane ethoxylate (14/3 EO/OH) triacrylate was used as the cross-linking agent.Resins of two different porosities were synthesized.The permeability to biological macromolecules was investigated by sizeexclusion chromatography,using the resin beads as the stationary phase.One of the resins was able to retard thyroglobulin (669 kDa) while the other,prepared with a lower amount of porogen,showed a lower fractionation range (1-60 kDa).
展开▼
机译：CLEAR载体最初是为固相合成而开发的。在这项研究中，使用甲苯作为成孔剂，通过自由基悬浮聚合制备了两种宽孔形式的树脂。使用三羟甲基丙烷乙氧基化物（14/3 EO / OH）三丙烯酸酯合成了两种不同孔隙率的树脂。以树脂珠为固定相，通过尺寸排阻色谱法研究了对生物大分子的渗透性。一种树脂能够阻滞甲状腺球蛋白（669 kDa），另一种能够阻滞甲状腺球蛋白（669 kDa）。用较少量的成孔剂制备，显示出较低的分馏范围（1-60 kDa）。
展开▼
