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Seed longevity in oilseed rape (Brassica napus L.) - genetic variation and QTL mapping

机译:油菜(Brassica napus L.)的种子寿命-遗传变异和QTL定位

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Although oilseed rape has become one of the most important oil crops in Europe, little is known regarding the viability of its seed under conditions of long-term storage. We report here an examination of oilseed rape seed longevity performed on a set of 42 accessions housed at the German ex situ genebank at IPK, Gatersleben. A comparison of germination between the accessions stored for 26 years showed that viability was in part genetically determined, since it ranged between 42 and 98%. An attempt was made to define the genetic basis of viability by subjecting a mapping population of doubled haploids to three artificial ageing treatments. Quantitative trait loci (QTL) were detected on six chromosomes: N6, N7, N8, N15, N16 and N18. The chromosomal locations of these QTL were compared with their syntenic regions in Arabidopsis thaliana in order to explore what genes might underlie genetic variation for longevity.
机译:尽管油菜已成为欧洲最重要的油料作物之一,但对其种子在长期储存条件下的生存能力知之甚少。我们在此报告了对一组42份油菜籽寿命的检验,这些油菜存放在位于Gatersleben IPK的德国易地基因库中。对保存26年的种质之间发芽的比较表明,生存力部分是基因决定的,因为其活力介于42%和98%之间。试图通过对双倍单倍体的作图群体进行三种人工老化处理来定义生存力的遗传基础。在六个染色体上检测到数量性状基因座(QTL):N6,N7,N8,N15,N16和N18。将这些QTL的染色体位置与拟南芥中的同义区域进行了比较,以探讨哪些基因可能是长寿遗传变异的基础。

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