A molecular study of dormancy breaking and germination in seeds of Trollius ledebouri.

摘要

A cDNA library was generated from seeds of Trollius ledebouri [T. chinensis] cv. Golden Queen after GA3 treatment. Five clones encoded mRNAs which were down-regulated during dormancy breaking and the initial stages of germination. Two of these showed homology to storage proteins (pPCB3 and pPCB4) and one each to the late-embryogenesis-abundant (LEA) group 2 dehydrin proteins (pPCB2), a barley glucose dehydrogenase (pPCB6) and the glutathione S-transferase (GST) superfamily (pPCB7). Transcript levels declined over 8 days in GA3-treated seeds. In dormant imbibed seeds transcript levels were relatively unchanged over the same period, except for transcripts of PCB3 which increased. Nucleotide sequence data for PCB2, PCB6 and PCB7 have been submitted to the EMBL/GenBank/DDBJ databases under the accession numbers Z69994 to Z69996, respectively.