AN S-RNASE PROMOTER FROM NICOTIANA ALATA FUNCTIONS IN TRANSGENIC N-ALATA PLANTS BUT NOT NICOTIANA TABACUM

摘要

Nicotiana tabacum and Nicotiana alata plants were transformed with genomic clones of two S-RNase alleles from N. alata. Neither the S-2 clone, with 1.6 kb of 5' sequence, nor the S-6 clone, with 2.8 kb of 5' sequence, were expressed at detectable levels in transgenic N. tabacum plants. In N. alata, expression of the S-2 clone was not detected, however the S-6 clone was expressed (at low levels) in three out of four transgenic plants. An S-6-promoter-GUS fusion gene was also expressed in transgenic N. alata but not N. tabacum. Although endogenous S-RNase genes are expressed exclusively in floral pistils, the GUS fusion was expressed in both styles and leaves. [References: 45]