Improved abiotic stress tolerance of bermudagrass by exogenous smallmolecules

摘要

As a widely used warm-season turf-grass in landscapes and golf courses,bermudagrass encounters multiple abi-otic stresses during the growth and devel-opment. Physiology analysis indicatedthat abiotic stresses induced the accumu-lation ofROS and decline of photosyn-thesis, resulting in increased cell damageand inhibited growth. Proteomic andmetabolomic approaches showed thatantioxidant enzymes and osmoprotectantcontents (sugar, sucrose, dehydrin, pro-line) were extensively changed under abi-otic stress conditions. Exogenousapplication of small molecules, such asABA, NO, CaCl2,H2S, polyamine andmelatonin, could effectively alleviatedamages caused by multiple abioticstresses, including drought,salt, heat andcold. Based on high through-put RNAseq analysis, genes involved in ROS, tran-scription factors, hormones, and carbo-hydrate metabolisms were largelyenriched. The data indicated that smallmolecules induced the accumulation ofosmoprotectants and antioxidants, keptcell membrane integrity, increased pho-tosynthesis and kept ion homeostasis,which protected bermudagrass fromdamages caused by abiotic stresses.