Establishment of an efficient and rapid method ofmultiple shoot regeneration and a comparativephenolics profile inin vitroandgreenhouse-grown plants ofpsophocarpustetragonolobus(L.)

摘要

Anin vitromethod of multiple shoot induction and plant regeneration inPsophocarpus tetragonolobus(L.) DC wasdeveloped. Cotyledons, hypocotyls, epicotyls, internodal and young seedling leaves were used as explants. MS mediasupplemented with various concentrations of either thidiazuron (TDZ) or N6-benzylaminopurine (BAP) along with NAAor IAA combinations were used to determine their influence on multiple shoot induction. MS media supplemented withTDZ induced direct shoot regeneration when epicotyls andinternodal segments were used as explants. TDZ at 3 mgL1induced highest rate (89.2§3.28%) of regeneration with (13.4§2.04) shoots per explant. MS media supplementedwith BAP in combination with NAA or IAA induced callus mediated regeneration when cotyledons and hypocotyls wereused as explants. BAP (2.5 mg L1) and IAA (0.2 mg L1) induced highest rate (100§2.66%) of regeneration with (23.2§2.66) shoots per explant. Mature plants produced from regenerated shoots were transferred successfully to thegreenhouse. In a comparative study, the phenolics contents of various parts of greenhouse-grown plants with that ofinvitro-raised plants showed significant variations.