Puroindoline genes are highly conserved in diploid ancestor wheats and related species but absent in tetraploid Triticum species.

摘要

A study was conducted to determine whether puroindoline-like genes are present in wheats of different ploidy levels and in other cereals. Polymerase chain reaction (PCR) experiments were conducted on genomic DNA isolated from diploid, tetraploid andhexaploid wheats, and from other cereals. The PCR approach showed that puroindoline genes are present in diploid and hexaploid Triticum species but absent in tetraploid species, and that T. tauschii [Aegilops tauschii] is likely to be the donor of the T. aestivum puroindoline genes. Puroindoline-like genes are present in cereals closely related to wheat (barley, oat and rye) and absent in cereals more distantly related to wheat (maize, rice and sorghum). Barley, oat and rye puroindoline-like sequencesand primary structure of deduced proteins are highly conserved. In oat, avenoindolines are more closely related to puroindolines than to tryptophanins. EMBL accession numbers for the sequences reported in this article are: AJ249929 (pHv71), AJ249928 (pHv111), AJ249930 (pAs52), AJ249931 (pAs3), AJ249932 (pSc64), AJ249933 (pTm31), AJ249934 (pTm83), AJ249935 (pTt11) and AJ249936 (pTt93).