Production of doubled-haploid plants from Lilium longiflorum Thunb. anther culture

摘要

The effects of different media, light conditions, andpretreatments by cold, and by centrifugation were evaluated on Liliumlongiflorum Thunb. anther culture. A high percentage of respondinganthers was achieved with N6 medium, by cold pretreatment and underdark conditions. Callus induction was promoted under darkness, butwas inhibited by light. Anthers from flower buds between 30 and 46 mmin length containing early- and mid-uninucleated microspores producedcallus. A total of 132 plantlets with bulblet and leaves wereregenerated. Among anther-callus regenerated plants no albino ormorphological variant was observed. The presence of both haploid anddiploid cells in the same root tip of regenerated plants indicatedthat chromosome doubling may have occurred spontaneously.