Production of doubled-haploid plants from Lilium longiflorum Thunb. anther culture

摘要

The effects of different media, light conditions, and pretreatments by cold, and by centrifugation were evaluated on Lilium longiflorum Thunb. anther culture. A high percentage of responding anthers was achieved with N6 medium, by cold pretreatment and under dark conditions. Callus induction was promoted under darkness, but was inhibited by light. Anthers from flower buds between 30 and 46 mm in length containing early- and mid-uninucleated microspores produced callus. A total of 132 plantlets with bulblet and leaves were regenerated. Among anther-callus regenerated plants no albino or morphological variant was observed. The presence of both haploid and diploid cells in the same root tip of regenerated plants indicated that chromosome doubling may have occurred spontaneously.