Production of Taxol registered trade mark sign and baccatin III by a selected Taxus baccata callus line and its derived cell suspension culture

摘要

Small callus pieces excised from a selected 1-year-old stable callus line of Taxus baccata were grown on B5 solid medium supplemented with either phenylalanine (1 mM) or the elicitor compound VSO inferior 4 (0.05 or 0.1 mM). Under these conditions, callus growth and the production of Taxol registered trade mark sign and baccatin III were tested during 8 weeks of culture. Growth was enhanced by the presence of both phenylalanine and VSO inferior 4. The elicitor compound VSO inferior 4 (0.05 and 0.1 mM) moreover clearly increased Taxol registered trade mark sign and baccatin III production, especially at the end of the culture. The specific activity of PAL in callus pieces cultured under the different conditions studied, is also considered. In addition, liquid cultures were prepared from the selected callus line in order to study the effect of the VSO inferior 4 addition to the culture medium on Taxol registered trade mark sign and baccatin III production. The presence of this elicitor (VSO inferior 4; 0.05 mM), increased Taxol registered trade mark sign and baccatin III production (by a factor of 2.5 and 3.6, respectively) from 5.2 mu g g superior - superior 1 DW to 13.1 mu g g superior - superior 1 DW for Taxol registered trade mark sign and from 4.4 mu g g superior - superior 1 DW to 16.0 mu g g superior - superior 1 DW for baccatin III in the T. baccata cell suspension cultures. It also increased their release into the medium by a factor of 3.2 and 2.2, respectively.