Agrobacterium-mediated high frequency transformation of an elite indica rice variety Pusa Basmati 1 and transmission of the transgenes to R2 progeny

摘要

Among rice, indica varieties are considered recalcitrant to tissue culture and genetic manipulation. We report here Agrobacterium-mediated transformation of an economically important, elite indica variety Pusa Basmati 1. A large number of morphologically normal and fertile transgenic plants have been obtained. Molecular and genetic analysis of transgenic plants reveals the integration, expression and inheritance of transgenes in the progeny of these plants. Twenty seven percent plants contain single copy gene insertion and copy number of transgenes has been found to vary from 1-4 in transgenic plants. Mendelian as well as non-Mendelian inheritance patterns of the introduced genes have been obtained in the R1 progeny.