Identification and expression of the GTcyc cDNA clone from genetic tumors formed on a Nicotiana glauca-N-langsdorffii hybrid

摘要

Cell division is one of the fundamental processes in all eukaryotes and plays a crucial role in growth and development of multicellular organisms. A 192-bp predicted DNA product was amplified from the genetic tumors of Nicotiana glanca-Nicotiana langsdorfii hybrid using degenerate primers specific for cyclin genes. A full-length cyclin cDNA clone was subsequently identified from the genetic tumors using a cDNA library screening strategy. In situ hybridization analysis showed that the GTcyc was detected in numerous, small spots in the whole range of genetic tumor. (C) 2000 Editions scientifiques et medicales Elsevier SAS. [References: 27]