Production of non- organogenic and loosely attached callus in leaf disk cultures of haploid Nicotiana plumbaginifolia by ~(14)N ion beam irradiation

摘要

The leaf disks of Nicotiana plumbaginifolia were irradiated by an ~(14)N heavy-ion beam and then were cultured in shoot-inducing medium in order to produce mutants for elucidating the functions of the cell wall in plant morphogenesis. Non-organogenic and loosely attached callus, which had the same morphological features as non-embryogenic callus of carrot was formed on 11.8% of the haploid leaf disks irradiated by ~(14)N heavy-ion beam (5 Gy) at high frequency. The cells stimulated by the heavy-ion beam loosely attached to each other resulting in a random morphology of cell clusters, whereas the non-irradiated cells formed a tight callus with multiple shoots.