In vitro plant regeneration from leaf callus of Grindelia robusta Nutt.

摘要

A regeneration protocol from leaf explants of Grindelia robusta Nutt. was developed. The combination of 0.5 mg l^-1 IBA plus 0.5 mg l^-1 or 1 mg l^-1 BA added to Murashige-Skoog (MS) medium resulted in the best callus induction frequency; the combination of 0.4 or 0.9 mg l^-1 BA plus 1.2 mg l^-1 GA₃ resulted in the best shoot regeneration. Rooting was successful on MS medium supplemented with 0.5 mg l^-1 IBA. Hardening of G. robusta plants was accomplished in 30 days with 85% survival rate.