Inheritance of resistance to iron deficiency and identification of AFLP markers associated with the resistance in mungbean (Vigna radiata (L.) Wilczek)

摘要

Iron deficiency chlorosis (IDC) is a major problem reducing yield of mungbean in many countries. In this study, we crossed KPS1, the most popular Thai mungbean cultivar susceptible to IDC with NM10-12, a mungbean line from Pakistan resistant to IDC. Segregation analysis of the F population revealed that the resistance is controlled by a major gene (IR) with dominant effect. Two AFLP markers, E-ACT/M-CTA and E-ACC/M-CTG were identified closely linking with the IR gene. The frequencies of these markers were assessed in 241 mungbean accessions from several countries. The accessions could be divided, in relative to total chlorophyll content of the resistant check (NM10-12) and the susceptible check (KPS1), into the resistant group with 125 accessions and the susceptible group with 116 accessions. Among 125 resistant accessions, E-ACT/M-CTA and E-ACC/M-CTG were present in 119 (95%) and 109 (87%) accessions, respectively. Both markers can identify all resistant accessions from England, Indonesia and Pakistan, but only E-ACT/M-CTA linked to all resistant accessions from Australia, India, Iraq, Taiwan and Thailand. Understanding the inheritance and identifying molecular markers linking to the IR gene can help plant breeders to improve this crop for growing in iron-deficient soils.