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Journeys through aroma space: a novel approach towards the selection of aroma-enriched strawberry cultivars in breeding programmes.

机译:穿越香气空间的旅程:一种在育种计划中选择富含香气的草莓品种的新颖方法。

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摘要

Consumers often criticize the sensory quality of modern strawberry cultivars. Therefore, a new and fast workflow for cultivar selection was developed to aid in the development of cultivars with enhanced aroma. This workflow consists of Headspace (HS) solid-phase microextraction (SPME) fast GC-MS used for high-throughput aroma analysis of parents and hybrids. For data analysis, a chemometrical workflow was created. With a principal component analysis (PCA), the aroma similarity between the samples and a target aroma was evaluated. In order to know how the aroma profiles of the parents and hybrids are situated towards this target aroma, the Euclidean distances were calculated. These were then further used for a partial least-squares (PLS) regression analysis to determine which aroma compounds are responsible for the observed distance to the target. By using this new approach, hybrids showing aroma profiles similar to the target aroma can be identified as most suitable candidates for further breeding cycles. Besides being time-saving, the suggested workflow introduces aroma analysis as an integral part of breeding programmes. It prevents losing the coherence within the aroma profile and subsequently the loss of important information
机译:消费者经常批评现代草莓品种的感官品质。因此,开发了一种新的,快速的品种选择工作流程,以帮助开发具有增强香气的品种。该工作流程由用于父母和杂种的高通量香气分析的顶空(HS)固相微萃取(SPME)快速GC-MS组成。为了进行数据分析,创建了化学计量工作流程。通过主成分分析(PCA),评估了样品与目标香气之间的香气相似性。为了知道亲本和杂种的香气特征如何朝向该目标香气定位,计算了欧几里德距离。然后将它们进一步用于偏最小二乘(PLS)回归分析,以确定哪些香气化合物负责观察到的目标距离。通过使用这种新方法,可以将显示出类似于目标香气的香气特征的杂种鉴定为进一步育种周期的最合适候选者。除了节省时间外,建议的工作流程还将香气分析作为育种计划的组成部分。它可防止失去香气内的连贯性,避免随后丢失重要信息

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