A cellulase free, alkaline, thermo-tolerant pectinase was produced by a novel yeast strain Pseudozyma sp. SPJ using citrus peel as inexpensive carbon source. The crude enzyme showed good prospects in degumming of flax fibers for textile industry. An optimum pectinase dose of 80 U g−1 resulted in reduction of 15 ± 1.92% dry weight of the fibers, releasing maximum galacturonic acid (10825.5 ± 34.2 μg g−1 dry fiber) after the incubation of 6 h. The yeast culture could grow on the flax fibers (as sole carbon source) without addition of any other nutrient and produce good enzyme yield (9235.5 ± 21.51 U g−1 dry fiber). After 12 h incubation of the fibers with the isolated yeast strain, 4471 ± 19.5 μg g−1 dry fiber galacturonic acid was achieved with maximum weight loss of 11 ± 1.2%. This process reduced the amount of chemicals and energy used in conventional methods. It also contributed to enhance fineness and overall quality of the fiber strands. This study is relevant to the textile industry as it provided a fast, economical and eco-friendly method for degumming of flax fibers.
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机译：一种新型酵母菌株假单胞菌（Pseudozyma sp。）产生了无纤维素酶的碱性耐热果胶酶。使用柑橘皮作为廉价碳源的SPJ。粗酶在纺织工业亚麻纤维的脱胶中显示出良好的前景。果胶酶的最佳剂量为80 U g -1 可使纤维干重减少15±1.92％，释放出最大的半乳糖醛酸（10825.5±34.2μgg -1 温育6小时后。酵母培养物可以在亚麻纤维（作为唯一碳源）上生长，而无需添加任何其他养分并产生良好的酶产量（9235.5±21.51 U g -1 干纤维）。将纤维与分离的酵母菌株孵育12小时后，获得4471±19.5μgg -1 干纤维半乳糖醛酸，最大失重为11±1.2％。此过程减少了常规方法中使用的化学药品和能源。它还有助于提高纤维股的细度和整体质量。这项研究与纺织工业有关，因为它为亚麻纤维的脱胶提供了一种快速，经济和环保的方法。
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