Development of Methodology for Concurrent Maximum Production of Alkaline Xylanase-Pectinase Enzymes in Short Submerged Fermentation Cycle

摘要

The aim of this study is to enhance the production of both enzymes concurrently in short fermentation cycle. In this study, effect of crude xylan and pectin substrates extracted from agro-residues, for the cost-effective, maximum concurrent production of xylanase-pectinase enzymes under submerged fermentation conditions was investigated. Crude xylan and crude pectin were extracted under alkaline and acidic conditions by using destarched, grinded wheat bran and citrus peel agro-wastes respectively. In order to make the production process more economical, different combinations with extracted substrates were tried for getting the maximum concurrent activity of both enzymes by reducing bacterial lag phase. Maximum alkaline xylanase activity of 296 ± 13 IU/ml and alkaline pectinase activity of 93 ± 7.0 IU/ml was achieved after 48 h of incubation, using production medium containing 2% wheat bran and 0.7% of crude pectin along with peptone, 0.5%; MgSO_4, 2.45%; pH 7.0 and 2% inoculum of 21 h old culture. This is the first report mentioning the utilization of agro-waste extracted pectin for enhanced production of pectinase in short incubation time, concurrently with xylanase.