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Canine Transmissible Venereal Tumour: Asessment of Mast Cell Numbers as Indicators of the Growth Phase

机译:犬可传播的性腺肿瘤:肥大细胞数量的评估作为生长期的指标

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Mast cells are immune cells that are involved mainly in type 1 hypersensitivity reactions, and they have been implicated in tumour angiogenesis. In this study we assessed the presence of mast cell numbers and microvessel density during the progression and regression stages of natural spontaneous canine transmissible venereal tumours (CTVT). Mast cells were demonstrated by histochemical staining with toluidine blue, alcian blue and safranin O. Microvessel counts were demonstrated by immunohistochemical labelling with an antibody against the endothelial cell marker factor VIII. Mitotic cells, apoptotic cells and tumour infiltrating lymphocytes were counted from haematoxylin–eosin-stained sections. Tumour fibrosis was evaluated on Masson's trichome-stained sections. The results showed that progressing tumours had significantly higher mast cell counts and microvessel counts at the invasive edges of the tumours than did regressing tumours. In both the progressing and regressing tumours, microvessel counts were significantly positively correlated with mast cell counts. Regressing tumours had significantly higher mast cell counts of the whole tumour than progressing tumours. The results also showed that progressing tumours had significantly higher mitotic rate than regressing tumours, and fibrosis and apoptosis were significantly higher in regressing tumours than progressing tumours. There were no significant differences between the biochemical and haematological values of dogs with progressing and regressing tumours. These results suggests that mast cells play a role in CTVT progression probably by promoting vascularization at the invasion front during the progression phase, and that mast cell count could be used as one of the histological factors to indicate growth stage of CTVT.
机译:肥大细胞是主要参与1型超敏反应的免疫细胞,它们与肿瘤血管生成有关。在这项研究中,我们评估了自然自发犬可传播性病(CTVT)的进展和消退阶段肥大细胞数量和微血管密度的存在。肥大细胞通过甲苯胺蓝,阿尔辛蓝和藏红素O的组织化学染色证明。微血管计数通过免疫组织化学标记抗内皮细胞标记因子VIII的抗体证明。从苏木精-伊红染色的切片中计数有丝分裂细胞,凋亡细胞和肿瘤浸润淋巴细胞。在Masson的毛状体染色切片上评估了肿瘤纤维化。结果表明,进展期肿瘤在肿瘤浸润边缘的肥大细胞计数和微血管计数显着高于消退肿瘤。在进展和消退的肿瘤中,微血管计数与肥大细胞计数显着正相关。消退性肿瘤的整体肥大细胞计数明显高于进行性肿瘤。结果还表明,进展中的肿瘤的有丝分裂率显着高于进展中的肿瘤,而纤维化和凋亡明显高于进展中的肿瘤。肿瘤进展和消退的狗的生化和血液学价值之间无显着差异。这些结果表明,肥大细胞可能通过促进进展阶段的侵袭前沿处的血管形成而在CTVT进展中起作用,并且肥大细胞计数可用作指示CTVT生长阶段的组织学因素之一。

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