Bioactive substance secreted by canine transmissible venereal tumor with mammalian B lymphocyte toxicity

摘要

The present invention herein relates to a bioactive substance with mammalian B lymphocyte toxicity secreted by canine transmissible venereal tumor (CTVT) and its process. CTVT is the only mammalian tumor in nature that is transmitted through viable tumor cells. Transmission of CTVT between dogs is akin to an allograft. The isolation of bioactive substance secreted by CTVT includes the following steps: (1) store CTVT excised from canine skin in Hank's balanced salt solution (HBSS); (2) physically extract CTVT, tumor infiltrating lymphocytes and peripheral blood lymphocytes; (3) place CTVT in culture medium and physically obtain suspension fluid with B lymphocyte toxicity; (4) drive said suspension solution through protein filters with different pore sizes to obtain bioactive substance. Said bioactive substance secreted by CTVT and having specific toxicity for mammalian B lymphocytes is a protein substance with a molecular weight between 30˜100 kD and free of &agr;-Dmannosyl and &agr;-D-glucosyl residues and it is soluble, heat-sensitive and proteinase K-sensitive.