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Detection of intercellular adhesion genes and biofilm production in Staphylococcus aureus isolated from bovine subclinical mastitis

机译:牛亚临床乳腺炎分离的金黄色葡萄球菌中细胞间粘附基因的检测和生物膜的产生

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Biofilm production by Staphylococcus aureus, an important virulence factor was investigated employing phenotypic and genotypic methods. A total of 102 S. aureus isolates from bovine subclinical mastitis cases were included in the study. Maximum number of biofilm producing strains were detected by Congo red agar (CRA) method (48.03%) followed by tube method (36.27%). Tissue culture plate method (TCP) without and with destaining identified 19.60 and 29.41% of S. aureus as biofilm producers, respectively. A polymerase chain reaction for detection of intercellular adhesion genes, icaA and icaD, responsible for biofilm formation was standardized. Of the 102 S. aureus isolates investigated, 36 (35.29%) strains revealed presence of both the genes. Considering polymerase chain reaction as a standard test, CRA and TCP without destaining were the most sensitive and specific, respectively. PCR technique standardized for detection of the icaA and icaD genes is reliable for identifying biofilm producing potential of S. aureus which may help in rapid detection of biofilm-producer Staphylococci. This would allow the early application of control measures. Keywords Staphylococcus aureus - Biofilm production - Mastitis - icaA - icaD
机译:使用表型和基因型方法研究了由金黄色葡萄球菌(一种重要的致病因子)产生的生物膜。该研究共包括来自牛亚临床乳腺炎病例的102株金黄色葡萄球菌。刚果红琼脂(CRA)方法(48.03%),然后是试管法(36.27%)检测到最大数量的生物膜生产菌株。不带和带脱色的组织培养板法(TCP)分别确定了金黄色葡萄球菌的19.60%和29.41%为生物膜生产者。用于检测细胞间粘附基因icaA和icaD的生物膜形成的聚合酶链反应已标准化。在所研究的102株金黄色葡萄球菌中,有36株(35.29%)显示出这两个基因的存在。以聚合酶链反应为标准测试,不加修饰的CRA和TCP分别是最敏感和最特异的。标准化的检测icaA和icaD基因的PCR技术可可靠地鉴定出金黄色葡萄球菌的生物膜生产潜力,这可能有助于快速检测生物膜生产者葡萄球菌。这样可以及早采取控制措施。关键词金黄色葡萄球菌-生物膜生产-乳腺炎-icaA-icaD

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