Overexpressions of lambda phage lysis genes and biosynthetic genes of poly-β-hydroxybutyrate in recombinant E. coli

摘要

A plasmid (pTu9) containing the lambda (λ) phage lysis genes S(−) RRz and the biosynthetic genes phb CAB of poly-β-hydroxybutyrate (PHB) was constructed and transformed into E. coli JM109. Oultured in Luria Bertani (LB) medium with 20g/Lglucose, E. coli JM109 (pTu9) could accumulate PHB in cells up to 40% (g PHB per g dry cells). Achelating agent EDTA was applied to induce a corrplete cell lysis and PHB granules were released. This method has a potential application in PHB separation.