Genetic assessment of eight Corchorus spp. (Tiliaceae) using RAPD and ISSR markers

摘要

A comparison of the efficiency of RAPD and ISSR primers using different genetic parameters like polymorphism percentage, effective alleles per locus (Aep), genetic diversity per locus (Hep), Shannon diversity index (I), polymorphic information content (PIC) and marker index (MI) has been performed in 8 Indian grown Corchorus spp. (2n = 14) of the family Tiliaceae (C. capsularis L., C. olitorius L., C. aestuans L., C. fascicularis Lamk., C. pseudocapsularis L., C. pseudoolitorius I. and Z., C. tridens L. and C. trilocularis L.). The objective of the work is to develop a simple, efficient and cost effective primer based system for quick genetic evaluation of Corchorus germplasms. Result indicated that OPA 03, OPA 05, OPA 06, OPB 03, OPB 06, OPC 05 and OPC 10 among the employed RAPD markers and (GA)12, (CA)8GC and (GATA)4 among ISSR primers are efficient and effective. Further, relatedness between the species has also been noted using principal component analysis (PCA) and cluster analysis by Unweighted Pair Group Method with Arithmetic Mean (UPGMA) with an objective for efficient breeding and crop improvement. Unrooted phylogenetic tree constructed from molecular data of the studied species revealed possible divaricated mode of origin of Corchorus.