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Genetic linkage map construction for white jute (Corchorus capsularis L.) using SRAP, ISSR and RAPD markers

机译:利用SRAP,ISSR和RAPD标记构建白麻(Corchorus capsularis L.)的遗传连锁图谱

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摘要

White jute (Corchorus capsularis) and dark jute (Corchorus olitorius) are two important cultivated crops that are used for natural fibre production. Some genetic maps have been developed for dark jute, but the genetic map information for white jute (C.capsularis) is limited. In this study, a linkage map comprising 44 sequence-related amplified polymorphisms (SRAPs), 57 intersimple sequence repeats (ISSRs) and 18 randomly amplified polymorphic DNA (RAPD) covering 2185.7cM with a mean density of 18.7cM per locus was constructed in an F-2 population consisting of 185 individuals derived from a cross between two diverse genotypes of Xinxuan No. 1' and Qiongyueqing' in white jute. These markers were evenly distributed in the linkage groups without any clustering. This genetic linkage map construction will facilitate the mapping of agronomic traits and marker-assisted selection breeding in white jute.
机译:白麻(Corchorus荚ris)和黑麻(Corchorus olitorius)是用于天然纤维生产的两种重要的栽培作物。已经开发了一些针对黑黄麻的遗传图谱,但是有关白麻黄(C.capsularis)的遗传图谱信息有限。在这项研究中,构建了一个包含44个序列相关的扩增多态性(SRAP),57个单序列重复序列(ISSR)和18个随机扩增的多态性DNA(RAPD)的连锁图,覆盖了2185.7cM,每个位点的平均密度为18.7cM。 F-2种群由185个个体组成,这些个体来自白麻黄新轩1'和琼月清'两种不同基因型之间的杂交。这些标记均匀地分布在链接组中,没有任何聚类。这种遗传连锁图谱的构建将有助于对白麻黄中农艺性状和标记辅助选择育种的作图。

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