Magnetically treated water at 4 Hz and 2.5 mT as a modulator of cisplatin effect on cell hydration and ouabain binding of sarcoma-180 tissue

摘要

There are many data about the extremely low-frequency electromagnetic fields (ELF-EMF) therapeutic use, especially in the field of oncology. Recent data suggest that 4 Hz EMF having dehydration effect on tissues has a pronounced antitumor activity on sarcoma-180 in mice. It was shown that 4 Hz EMF have pronounced effects on physicochemical properties of water and water solution. Therefore, the aim of the present work was the comparative study of the modulation effect of ELF-EMF on cisplatin-induced changes cell hydration and number of ouabain receptors in membrane of sarcoma-180 tumor tissues. Tissue hydration was measured as wet weight/dry weight and expressed as a water content of g/g in dry weight. The number of ~3H-ouabain receptors in membrane was counted by isotope scintillation counter. In conclusion, ELF-EMF can be a possible tool for stimulation of cisPt antitumor effect.