Application of platinum nanoparticles as affinity probe and matrix for direct analysis of small biomolecules and microwave digested proteins using matrix-assisted laser desorption/ionization mass spectrometry

摘要

We report the use of platinum nanoparticles (PtNPs) for analysis of amino acids, peptides, proteins andnmicrowave digested proteins (lysozyme and bovine serum albumin) without any tedious washing andnseparation procedures prior to matrix-assisted laser desorption/ionization time-of-flight massnspectrometry (MALDI-TOF-MS). In the present study, PtNPs play three functions, such as matrix,naffinity probe and acceleration of protein digestion by absorbing the microwave irradiation. Goodnsignal intensity of the target molecules from the sample was obtained when laser energy, NPsnconcentration and incubation time were set to 35 mJ, 25 nM and 30 min, respectively. In addition,nhigher numbers of peptide sequence were obtained for microwave digested lysozyme protein usingnPtNPs as compared to previously reported methods for analysis of digested protein in MALDI-MS.nThus, the present method is a simple, rapid and one step preparation method for the analysis of aminonacids, peptides, proteins and digested proteins in MALDI-TOF-MS without the need for any tediousnpurifications and washing procedures.