Establishment of a local map of AFLP markers around the powdery mildew resistance gene Run1 in grapevine and assessment of their usefulness for marker assisted selection

摘要

The monogenic dominant genetic determinism of total resistance to powdery mildew, introduced from Muscadinia rotundifolia into Vitis vinifera, was further assessed in BC4 and BC5 full-sib families. A BC5 population of 157 individuals was used to select AFLP markers linked to the resistance gene, Run1. Thirteen AFLP markers were selected and a local map was constructed around the Run1 gene. Ten markers among the 13 were found to co-segregate with the resistance gene. The usefulness of these 13 AFLP markers for the selection of Run1-carrying genotypes was further assessed through their analysis in a set of 22 Run1-carrying resistant genotypes and 16 susceptible genotypes. Three markers out of the 13 analysed were found to be absent in all susceptible genotypes and present in all resistant individuals, and may thus represent good tools for the marker-assisted selection of grapevine varieties resistant to powdery mildew. A recombination event among the markers that were originally found to co-segregate was observed in one of the resistant individuals, showing that recombination is possible in this region and may therefore be observed in larger populations.