Pedigree analysis and haplotype sharing within diverse groups of Zea mays L. inbreds

摘要

The objectives of this study were the examination of genetic similarities in a diverse group of maize inbreds and an investigation of the incidence of shared haplotypes within closely related groups. Size polymorphisms from 218 mapped simple-sequence repeats (SSR) for 57 entries were detected with the ABI377 Prism system and scored with Genotyper software. The standard error for the estimated size of identical PCR products was 0.13 base pairs. Size estimates were used to examine genetic relationships among the Iodent, flint, corn belt dent, sweet corn and popcorn groups in Zea mays L. inbreds. Cluster analysis of SSR distance data from 57 entries showed similarity between the European flints (F2, F7 and EP1), CO109 and the su1 sweet corns developed in the United States. The inbred F64 from Argentina was distinct from all other entries. Close examination of two sources of B37 revealed that the Purdue University version of B37 contains a set of alleles characteristic of B73. Five groups (Iodents, European flints, the B73 group of corn belt dents, su1 sweet corn, popcorn) show persistent within-group haplotypes.