首页> 外文期刊>Theoretical and Applied Genetics >Development of a codominant PCR-based marker for allelic selection of the pink trait in onions (Allium cepa), based on the insertion mutation in the promoter of the anthocyanidin synthase gene
【24h】

Development of a codominant PCR-based marker for allelic selection of the pink trait in onions (Allium cepa), based on the insertion mutation in the promoter of the anthocyanidin synthase gene

机译:基于花青素合酶基因启动子中的插入突变,开发基于PCR的共轭标记,用于等位基因选择洋葱中的粉红色性状(葱属)

获取原文
获取原文并翻译 | 示例
       

摘要

Bulb color in onions (Allium cepa) is an important trait and is inherited in a complex manner. However, the mechanism of color inheritance is poorly understood at the molecular level. A previous study showed that pink bulb color in onions is inherited as a single recessive trait. This trait is attributable to a significantly reduced transcription of the anthocyanidin synthase (ANS) gene. In this study, we developed a PCR-based marker for an allelic selection of the ANS gene to avoid the laborious progeny tests traditionally employed. To identify polymorphisms between pink and red alleles of the ANS gene, promoter sequences of both alleles were isolated. There was 97% nucleotide sequence identity between the promoter sequences of the two alleles. A 390-bp insertion was identified 632 bp upstream from the putative transcription start site in the pink allele. A pair of primers was designed on the flanking sequences of the inserted region and utilized as a PCR-based marker for allelic selection of the ANS gene. The reliability of the marker was tested using parents, F1 hybrids, and F3 lines whose genotypes had been identified by progeny tests. The marker was also used to evaluate the distribution of the pink allele in white and yellow breeding lines. The results indicated that a majority of the breeding lines tested were homozygous recessive.
机译:洋葱(葱属洋葱)的鳞茎颜色是重要特征,并且以复杂的方式遗传。但是,在分子水平上对颜色遗传的机理了解甚少。先前的研究表明,洋葱中的粉红色鳞茎颜色是作为单个隐性性状遗传的。此特性可归因于花青素合酶(ANS)基因的转录明显减少。在这项研究中,我们开发了基于PCR的标记用于ANS基因的等位基因选择,从而避免了传统上繁琐的后代测试。为了鉴定ANS基因的粉红色和红色等位基因之间的多态性,分离了两个等位基因的启动子序列。两个等位基因的启动子序列之间有97%的核苷酸序列同一性。在粉色等位基因中假定的转录起始位点上游632 bp处鉴定到一个390 bp的插入。在插入区域的侧翼序列上设计了一对引物,并用作基于PCR的标记,用于ANS基因的等位基因选择。使用亲本,F1 杂种和F3 品系测试了标记物的可靠性,这些品系的基因型已通过后代测试鉴定。标记物还用于评估粉红色等位基因在白色和黄色育种系中的分布。结果表明测试的大多数育种系是纯合隐性的。

著录项

  • 来源
    《Theoretical and Applied Genetics》 |2005年第3期|573-578|共6页
  • 作者单位

    Vegetable and Fruit Improvement Center Department of Horticultural Sciences Texas AM University;

    Vegetable and Fruit Improvement Center Department of Horticultural Sciences Texas AM University;

    Vegetable and Fruit Improvement Center Department of Horticultural Sciences Texas AM University;

  • 收录信息
  • 原文格式 PDF
  • 正文语种 eng
  • 中图分类
  • 关键词

相似文献

  • 外文文献
  • 中文文献
  • 专利
获取原文

客服邮箱:kefu@zhangqiaokeyan.com

京公网安备:11010802029741号 ICP备案号:京ICP备15016152号-6 六维联合信息科技 (北京) 有限公司©版权所有
  • 客服微信

  • 服务号