...
首页> 外文期刊>Sensors Journal, IEEE >Plastic-Based Distributed Feedback Laser Biosensors in Microplate Format
【24h】

Plastic-Based Distributed Feedback Laser Biosensors in Microplate Format

机译:微孔板形式的塑料基分布式反馈激光生物传感器

获取原文
获取原文并翻译 | 示例
   

获取外文期刊封面封底 >>

       

摘要

A process that combines polymer nanoreplica molding with horizontal dipping was used to fabricate large area (~ 3 × 5 inch2) distributed feedback laser biosensors (DFBLB) on flexible plastic substrates, which were subsequently incorporated into standard format 96-well microplates. A room temperature nanoreplica molding process was used to create subwavelength periodic grating structures, while a horizontal dipping process was used to apply a ~ 300 nm, dye-doped polymer film. In this work, the DFBLB emission wavelength, used to characterize the device uniformity, demonstrated a coefficient of variation (CV) of 0.41% over the fabricated device area, representing a thickness standard deviation of only ~ 35 nm for the horizontal dipping process. The fabricated sensors were further characterized for sensitivity uniformity by measuring the bulk refractive index of the media exposed to the sensor surface and by measuring adsorption of biomolecular layers. An assay for detection of the cytokine Tumor Necrosis Factor-alpha (TNF-α) was used to demonstrate the operation of the sensor in the context of label-free detection of a disease biomarker. The demonstrated capability represents an important step towards roll-to-roll manufacturability for this biosensor that simultaneously incorporates high sensitivity with excellent wavelength shift resolution, and adaptability to the microplate format that is ubiquitous in pharmaceutical research.
机译:采用将聚合物纳米复制品成型与水平浸渍相结合的工艺,在柔性塑料基板上制造大面积(〜3×5英寸 2 )分布式反馈激光生物传感器(DFBLB),随后将其合并为标准格式96孔微孔板。室温纳米复制品模制工艺用于创建亚波长周期性光栅结构,而水平浸渍工艺用于施加〜300 nm的染料掺杂聚合物薄膜。在这项工作中,用来表征器件均匀性的DFBLB发射波长在整个器件面积上显示出0.41%的变异系数(CV),对于水平浸渍工艺而言,其厚度标准偏差仅为〜35 nm。通过测量暴露于传感器表面的介质的整体折射率并通过测量生物分子层的吸附,可以进一步对制造的传感器的灵敏度均匀性进行表征。一种检测细胞因子肿瘤坏死因子-α(TNF-α)的检测方法被用来证明传感器在疾病生物标记物的无标记检测中的操作。证明的能力代表了该生物传感器实现卷对卷可制造性的重要一步,该传感器同时兼具高灵敏度和出色的波长偏移分辨率,以及对制药研究中普遍使用的微孔板格式的适应性。

著录项

  • 来源
    《Sensors Journal, IEEE》 |2012年第5期|p.1174-1180|共7页
  • 作者

    Yafang Tan;

  • 作者单位
  • 收录信息
  • 原文格式 PDF
  • 正文语种 eng
  • 中图分类
  • 关键词

相似文献

  • 外文文献
  • 中文文献
  • 专利
获取原文

客服邮箱:kefu@zhangqiaokeyan.com

京公网安备:11010802029741号 ICP备案号:京ICP备15016152号-6 六维联合信息科技 (北京) 有限公司©版权所有
  • 客服微信

  • 服务号