Highly selective fluorescence enhancement chemosensor for Hg~(2+) based on rhodamine and its application in living cells and aqueous media

摘要

A rhodamine-based chemosensor (RP) was synthesized and characterized as a highly selective and sensitive probe for Hg2* in aqueous media. A prominent fluorescence enhancement at 576 nm was observed in the presence of Hg2*, accompanied by the change in the absorption spectrum. With the optimum condition described, the response was linearly proportional to the concentration of Hg~(2+) in the range 0-1 μM with a detection limit of 0.012 μM. Confocal laser scanning microscopy experiments showed that RP can sense the Hg~(2+) in living cells with a fluorescence enhancement signal. The proposed method was also successfully applied to the determination of Hg~(2+) in tap water, river water samples.