A novel bio-nanocomposite based on hemoglobin and carboxyl graphene for enhancing the ability of carrying oxygen

摘要

Hemoglobin (Hb) was successfully combined with carboxyl graphene (CG) through condensation reaction to form novel bio-nanocomposite hemoglobin-carboxyl graphene bio-nanocomposites (CG-Hb). Scanning electron microscope (SEM), X-ray diffraction (XRD), X-ray photoelectron spectroscopy (XPS), Fourier transform infrared spectroscopy (FTIR) and Raman spectra indicated that the morphology and structure of CG-Hb. Cyclic voltammograms of CG-Hb modified on the gold nanoparticles (Au-NPs) electro-deposited on glassy carbon electrode (GCE) exhibited a pair of redox peaks (-0.235 V and -0.122 V vs. SCE) and showed excellent biosensor for hydrogen peroxide, which realized the direct electron transfer between CG-Hb and GCE. The electrochemistry properties and the ability of oxygen-carrying were investigated by electrochemical methods. It was found this kind of bio-nanocomposite maintained the natural biological activity of Hb and was explored to be no observable denaturation, which can be considered as a novel effective oxygen carrier in electrochemical aspect for further research.