A bis-hydrazone derivative of 2,5-furandicarboxaldehyde with perfect hetero-atomic cavity for selective sensing of Hg(Ⅱ) and its intracellular detection in living HeLa S3 cell

摘要

A new bis-hydrazone derivative of 2,5-furandicarboxaldehyde (R1) was designed with perfect heteroatomic cavity and synthesized for sensing of heavy metal ion via FRET mechanism. Selective sensing ability of Rl as a lturn on' fluorescent chemosensor for Hg~(2+) with rapid and very sharp visual colour change was observed. The long wavelength fluorescence emission of R1:Hg~(2+) complex was applied for intracellular Hg~(2+) detection in living HeLa S3 cells. Single crystal X-ray confirmed the structure and LOD calculation suggested that the sensor could identify very low concentration (3.6 nM) of Hg~(2+). Quantum chemical (DFT) calculation supported the symmetrical three dimensional structural arrangements as well as the chelate complex of the sensor with Hg~(2+).