首页> 外文期刊>Science >Comprehensive Mapping of Long-Range Interactions Reveals Folding Principles of the Human Genome
【24h】

Comprehensive Mapping of Long-Range Interactions Reveals Folding Principles of the Human Genome

机译:远距离相互作用的综合作图揭示了人类基因组的折叠原理。

获取原文
获取原文并翻译 | 示例
       

摘要

We describe Hi-C, a method that probes the three-dimensional architecture of whole genomes by coupling proximity-based ligation with massively parallel sequencing. We constructed spatial proximity maps of the human genome with Hi-C at a resolution of 1 megabase. These maps confirm the presence of chromosome territories and the spatial proximity of small, gene-rich chromosomes. We identified an additional level of genome organization that is characterized by the spatial segregation of open and closed chromatin to form two genome-wide compartments. At the megabase scale, the chromatin conformation is consistent with a fractal globule, a knot-free, polymer conformation that enables maximally dense packing while preserving the ability to easily fold and unfold any genomic locus. The fractal globule is distinct from the more commonly used globular equilibrium model. Our results demonstrate the power of Hi-C to map the dynamic conformations of whole genomes.
机译:我们描述了Hi-C,一种通过将基于邻近度的连接与大规模平行测序相结合来探测整个基因组的三维结构的方法。我们用Hi-C构建了人类基因组的空间邻近图,其分辨率为1兆碱基。这些图证实了染色体区域的存在以及基因丰富的小染色体的空间邻近性。我们确定了一个附加水平的基因组组织,其特征在于开放和闭合染色质的空间分隔形成两个全基因组区室。在兆碱基规模上,染色质构象与分形小球一致,这是一种无结的聚合物构象,能够最大程度地密集堆积,同时保留了容易折叠和展开任何基因组基因座的能力。分形小球不同于更常用的球状平衡模型。我们的结果证明了Hi-C可以绘制整个基因组动态构象的能力。

著录项

  • 来源
    《Science》 |2009年第5950期|289-293|共5页
  • 作者单位

    Broad Institute of Harvard and Massachusetts Institute of Technology (MIT), MA 02139, USA Division of Health Sciences and Technology, MIT, Cambridge, MA 02139, USA Program for Evolutionary Dynamics, Department of Organismic and Evolutionary Biology, Department of Mathematics, Harvard University, Cambridge, MA 02138, USA Department of Applied Mathematics, Harvard University, Cambridge, MA 02138, USA;

    Program in Gene Function and Expression and Department of Biochemistry and Molecular Pharmacology, University of Massachusetts Medical School, Worcester, MA 01605, USA;

    Broad Institute of Harvard and Massachusetts Institute of Technology (MIT), MA 02139, USA;

    Division of Health Sciences and Technology, MIT, Cambridge, MA 02139, USA;

    Fred Hutchinson Cancer Research Center, Seattle, WA 98109, USA Department of Radiation Oncology, University of Washington School of Medicine, Seattle, WA 98195, USA;

    Fred Hutchinson Cancer Research Center, Seattle, WA 98109, USA Department of Radiation Oncology, University of Washington School of Medicine, Seattle, WA 98195, USA;

    Broad Institute of Harvard and Massachusetts Institute of Technology (MIT), MA 02139, USA;

    Program in Gene Function and Expression and Department of Biochemistry and Molecular Pharmacology, University of Massachusetts Medical School, Worcester, MA 01605, USA;

    Department of Genome Sciences, University of Washington, Seattle, WA 98195, USA;

    Department of Genome Sciences, University of Washington, Seattle, WA 98195, USA;

    Department of Genome Sciences, University of Washington, Seattle, WA 98195, USA;

    Broad Institute of Harvard and Massachusetts Institute of Technology (MIT), MA 02139, USA Department of Pathology, Harvard Medical School, Boston, MA 02115, USA;

    Department of Pediatrics, University of Washington, Seattle, WA 98195, USA;

    Fred Hutchinson Cancer Research Center, Seattle, WA 98109, USA Department of Radiation Oncology, University of Washington School of Medicine, Seattle, WA 98195, USA;

    Broad Institute of Harvard and Massachusetts Institute of Technology (MIT), MA 02139, USA;

    Department of Genome Sciences, University of Washington, Seattle, WA 98195, USA;

    Division of Health Sciences and Technology, MIT, Cambridge, MA 02139, USA Department of Physics, MIT, Cambridge, MA 02139, USA;

    Broad Institute of Harvard and Massachusetts Institute of Technology (MIT), MA 02139, USA Department of Biology, MIT, Cambridge, MA 02139, USA Department of Systems Biology, Harvard Medical School, Boston, MA 02115, USA;

    Program in Gene Function and Expression and Department of Biochemistry and Molecular Pharmacology, University of Massachusetts Medical School, Worcester, MA 01605, USA;

  • 收录信息 美国《科学引文索引》(SCI);美国《工程索引》(EI);美国《生物学医学文摘》(MEDLINE);美国《化学文摘》(CA);
  • 原文格式 PDF
  • 正文语种 eng
  • 中图分类
  • 关键词

  • 入库时间 2022-08-18 02:55:14

相似文献

  • 外文文献
  • 中文文献
  • 专利
获取原文

客服邮箱:kefu@zhangqiaokeyan.com

京公网安备:11010802029741号 ICP备案号:京ICP备15016152号-6 六维联合信息科技 (北京) 有限公司©版权所有
  • 客服微信

  • 服务号