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MAGNETIC BEADS

机译:磁珠

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摘要

MagSi-proteomics beads are magnetic beads that are an ideal tool for the purification, concentration, and desalting of peptides and protein digests. The surface of the beads has been modified with C4-, C8-, and C18-alkyl groups that are optimized for reversed phase applications. Magnetic beads offer a convenient solid support for a variety of assays and procedures based on affinity purification. They are especially well suited for automated procedures because instrumentation is available to easily mix, incubate, and separate the magnetic beads in 96-well plates without columns or centrifugation. Offering higher throughput and cost efficiency compared to tip-based separations, MagSi proteomics beads efficiently bind and elute even tiny sample fractions. The high magnetic strength of MagSi beads makes them applicable for both manual and automated/robotic frac-tionation, because the beads will typically collect in less than one minute when magnetic force is applied.
机译:MagSi蛋白质组学小珠是磁性小珠,是纯化和浓缩肽和蛋白质消化液的理想工具。珠的表面已被C4-,C8-和C18-烷基改性,这些烷基已针对反相应用进行了优化。磁珠为基于亲和纯化的各种测定和程序提供了方便的固体支持。它们特别适合自动化程序,因为仪器可轻松混合,孵育和分离96孔板中的磁珠,而无需色谱柱或离心分离。与基于尖端的分离相比,MagSi蛋白质组学珠具有更高的通量和成本效率,可有效地结合和洗脱甚至很小的样品部分。 MagSi磁珠的高磁性强度使其适用于手动和自动/机械破碎,因为当施加磁力时,磁珠通常会在不到一分钟的时间内收集。

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  • 来源
    《Science》 |2012年第6067期|p.481|共1页
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  • 收录信息 美国《科学引文索引》(SCI);美国《工程索引》(EI);美国《生物学医学文摘》(MEDLINE);美国《化学文摘》(CA);
  • 原文格式 PDF
  • 正文语种 eng
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  • 入库时间 2022-08-18 02:53:20

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