HUMAN ES/IPS MEDIUM

摘要

The new PluriSTEM Human ES/iPS Medium is designed for the routine expansion of human embryonic and induced pluripotent stem cells in feeder- and serum-free conditions with less frequent feeding and cell culture time. It is the first human ES/iPS media to combine small molecule inhibitors with pluripotent specific growth factors and supplements to enable a less rigorous cell culture regiment. The medium consists of fourteen components and is fully defined. The proprietary formulation includes Activin-A, TGFβ1, and b-FGF to promote stem cell self-renewal and potent small molecule combinations to inhibit unwanted spontaneous differentiation as well as human serum albumin (HSA) to aid in overall colony morphology. The culture of human ES/iPS cells has traditionally required feeding every day, including weekends, as well as significant technical expertise. This new medium addresses these challenges by providing an efficient and robust culture method that allows for feeding every other day, excluding weekends.