A new DGT technique comprised in a hybrid sensor for the simultaneous measurement of ammonium, nitrate, phosphorus and dissolved oxygen

摘要

A new diffusive gradients in thin films technique (ZrO-AT DGT) with zirconium oxide, A-62 MP and T-42H resins containing in a single binding gel was developed for simultaneous measurement of nitrate (NO_3-N), ammonium (NH_4-N) and phosphate (PO_4-P). The DGT uptake was found to be independent of pH variation from 3.2-8.7. Ionic strengths below 5,10 and 750 mmol·L~(-1) Nacl did not affect DGT uptake of NH_4-N. NO_3-N and PO_4-P, respectively. This new DGT was deployed in natural freshwater environments, with in situ measurements of the three nutrients found to be accurate. It ensured that rinsing the exposed surface of the DGT device at 3-day intervals can prevent biofouling. Additionally, a hybrid sensor comprising the novel DGT binding layer overlying an O_2 planar optrode was tested in sediments to evaluate the dynamics of O_2 and the three nutrients. Results showed that PO_4-P and NO_3-N fluxes decreased while fluxes of NH_4-N increased under aerobic conditions. Nearly simultaneous variation in O_2 and NO_3-N was observed at the sediment-water interface (SWI) and transformation of NO_3-N and PO_4-P was found to be sensitively influenced by O_2 dynamics.