Antioxidant and gene expression responses of Eisenia fetida following repeated exposure to BDE209 and Pb in a soil-earthworm system

摘要

This study first adopted repeated treatment model to investigate stress responses in earthworms (Eisenia fetida) following exposure to decabromodiphenyl ether (BDE209) and lead (Pb), which are the mainly co-existed contaminants at e-waste recycling sites. We evaluated the impacts of BDE209-Pb on antioxidative enzyme (superox-ide dismutase, SOD; catalase, CAT) activities, malondialdehyde (MDA) contents and transcriptional levels of three target genes (SOD, CAT and Hsp70), and further explored the relationships among these biomarkers. Results demonstrated that almost all the parameters were generally induced and the responses followed certain dose-effect relationships. Compared to the controls, a significant (P＜ 0.05) up-regulation trend of expression levels of the three genes could be clearly observed after 14 days incubation. Additionally, there existed good correlations between target genes expression levels and antioxidant enzyme activities (R ＞ 0.64). The observations could provide important information of ecotoxicological effects of BDE209-Pb in a soil-earthworm system as well as the mechanism of antioxidant defense.