Longitudinal assessment of prenatal phthalate exposure on serum and cord thyroid hormones homeostasis during pregnancy - Tainan birth cohort study (TBCS)

摘要

Highlights•Longitudinal Analysis of Urinary Phthalate Metabolite Levels at Three Trimesters during Pregnancy.•Repeat Measurement of Serum and Cord Thyroid Hormones in Pregnant Women.•Increased Maternal DBP and DEHP Exposure are Associated with Maternal T3,TSH, Free T4, and Cord Free T4.•Maternal DBP exposure during pregnancy is positively associated with maternal and cord free T4.•Different Exposure Windows of Phthalates during Pregnancy May be Crucial for Cord and Maternal Thyroid Hormone Homoeostasis.Graphical abstractDisplay OmittedAbstractAn increasing number of studies have revealed that phthalate exposure alters thyroid hormone homeostasis in the general population, but there is insufficient evidence of the effect of longitudinal maternal phthalate exposure on maternal and fetal thyroid hormones during pregnancy. We longitudinally assessed the effect of prenatal phthalate exposure in pregnant women on umbilical cord and maternal thyroid hormones at three trimesters during pregnancy. We recruited 98 pregnant women and collected urine and blood samples at three trimesters in an obstetrics clinic in Southern Taiwan from 2013 to 2014. We analyzed the concentrations of 11 urinary phthalate metabolites, including monoethylhexyl phthalate, mono-(2-ethyl-5-oxo-hexyl) phthalate (MEOHP), mono-(2-ethyl-5-hydroxyhexyl) phthalate, mono-(2-ethyl-5-carboxypentyl) phthalate (MECPP), mono-n-butyl phthalate, monoisobutyl phthalate (MiBP), monoethyl phthalate (MEP), using online liquid chromatography–tandem mass spectrometry. The cord and maternal serum levels of thyroxine (T4), free T4, triiodothyronine (T3), thyroid-stimulating hormone (TSH), and thyroxine-binding globulin were measured using an electrochemiluminescence immunoassay. A mixed-model analysis was utilized to assess the effect of longitudinal phthalate exposure on thyroid hormones and adjusted for significant covariates. We found that urinary MiBP (β=−0.065, 95% confidence interval (CI): −0.124, −0.005), and MEOHP (β=−0.083, 95% CI: −0.157, −0.009) were significantly negatively associated with serum TSH. Urinary MECPP was inversely related to serum T3(β=−0.027, 95% CI: −0.047, −0.006). Urinary MEP (β=0.014, 95% CI: −0.001, 0.028) and MiBP (β=0.033, 95% CI: 0.018, 0.049) were positively related to free T4. We found that cord serum T3(β=0.067, 95% CI: 0.003, 0.131) and free T4(β=0.031, 95% CI: 0.001, 0.062) levels had significant positive associations with maternal ΣDBPm levels at the second trimester. We concluded that different phthalates exposure windows during gestation may alter cord and serum thyroid hormone homoeostasis.