Alteration in concentration-response curves of four N-alkylpyridinium chloride by exposure concentration, time and in their mixtures by uniform design

摘要

The concentration-response curves (CRCs) of chemicals are important in extrapolating their effects from laboratory studies to their risk assessment in the field. Yet, the CRCs can be altered by exposure concentration and mixture conditions, and also by exposure time in recent reports. Presently, ionic liquids (N-alkylpyridinium chloride, [apyr]Cl) were used for CRC-alteration studies. In individual effects on Vibrio qinghaiensis sp. Q67 (067) from 0.25 to 24 h, the CRCs of [epyr]Cl and [bpyr]Cl changed from S- to J-shaped with decreases in inhibition and increases in stimulation, while the CRCs of [hpyr]Cl changed from S- to flat-shape with decreases in inhibition but without stimulation. In mixture effects on Q67, the CRCs all changed from S- to J-shaped from 0.25 to 24 h. By means of the variable selection and modeling method based on the prediction (VSMP), the CRC-alterations of mixtures were positively contributed by [epyr]Cl but negatively contributed by [bpyr]Cl. Furthermore, a parameter was developed by the area of a triangular that combined acute inhibition (EC_(50.0.25h)) and chronic stimulation (Zero-effect Point, i.e., ZEP_(24h) and the minimum inhibition effect, i.e., E_(min,24h)). This parameter successfully evaluated the CRC-alterations in both individual and mixture effects over time, and indicated potential interactions in CRC-alteration in mixtures.