Crystal structure of Streptomyces diastaticm No. 7 strain M1033 xylose isomerase

摘要

The crystal structures of Streptomyces diastaticus No. 7 strain M1033 xylose isomerase (SDXyl) have been analysed and refined at 0. 19 nm. The crystal space group is I222, with unit cell dimensions of a = 9.884 nm, 6 = 9.393 nm and c=8.798 nm. Based on the coordinates of the Streptomyces rubiginosus xylose isomerase (SRXyI), the initial model of SDXyI was built up by the close packing analysing and R-factor searching and refined by PROLSQ to a final R-factor of 0.177 with the. rms deviations of bond lengths and bond angles of 0.001 9 nm and 2.1°, respectively. No significant global conformation change existed between SRXyI and SDXyI except the local conformation in the active site.