Construction of the glucose isomerase deficient strain of Streptomyces M1033 by homologous recombination

摘要

After the establishment of the transformation conditions of Streptomces diastaticus No.7 Strain M1033, the integration plasmid pXW for homologous recombination, which contains a 6000 bp fragment of incomplete GI (GI138P. G247D) gene, has been constructed in order to realize the stable overexpression of the GI (G138. G247D) which is valuable for large-scale industrial production.