Analysis of genes specifically expressed under salt stress in salt-tolerant mutant of rice by using DDRT- PCR technique

摘要

Differential display reverse transcription-PCR (DDRT-PCR) technique was used to identify those genes that are expressed differentially between wild type rice variety 77-170 (Oryza Saliva var Japonica) and its salt-tolerant mutant (M-20) under salt stress. Totally 13 salt-inducible cDNA fragments of 200—600 bp were identified and cloned, and were designated as SIGR1 — SIGR13 (salt-induced gene in rice). Northern blot analysis showed that expression of SIGR6 and SIGR8 was salt-inducible in both wildtype and mutant, and expression of SIGR12 in M-20 Was much higher than that in 77-170 under salt stress. It was also shown that expression of SIGR3, SIGR4, SIGR7, SIGR10 and SIGR13 was salt-inducible, and the genes were highly homologous with Rabid whichwas an ABA-inducible gene of rice. The great potential application of DDRT-PCR technique in plant molecular biology research may promote the investigation of expression of salt-induced protein in rice.