In situ amplification of DNA fragments specific for human Y chromosome in cellular nuclei by PCR

摘要

Using single primer pairs Y_3 and Y_4, in situ polymerase chain reaction (in situ PCR) was successfully performed on the specimen slides of peripheral leukocytes. By both of the direct digoxiginin-11-dUTP incorporation into PCR products with in situ PCR (direct in situ PCR) and in situ PCR followed by detection of in situ hybridization (indirect in situ PCR), DNA fragments specific for human Y chromosome were obviously amplified in cellular nuclei of specimens on the slides. The results were verifiedby Southern analysis. The methodology of in situ PCR and its application were discussed.