Establishment of La-tPA/G-CSF dual trangenic mice and expression in their mammary gland

摘要

Expression vectors of human granulocyte colony stimulating factor(G-CSG)and long acting tissue plas- minogen activator(La-tPA)in mammary gland were constructed using promoters of mouse whey acid protein gene (WAP)and sheepβ-lactoglobulin gene(BLG)with sizes of 2.6 and 5 kd respectively. Two kinds of transgenic mice of G-CSF and La-tPA were produced with microinjection. The expression of G-CSF and La-tPA was achieved in mammary Glands of transgenic mice, respectively.