Protein Preparation, Crystallization and Preliminary X-Ray Crystallographic Studies of Smu.1392c from Streptococcus mutans

摘要

Smu.1392c is a protein with 158 residues of uncharacterized function. Bioinformatics studies predict it is a putative acetyltransferase. In order to identify its exact function via structural studies, Smu.1392c gene was amplified from Streptococcus mutans genomic DNA and cloned into expression vector PET28a. Smu.1392c was crystallized and diffracted to a resolution of 3 Å in-house. The crystal belongs to R32 space group, with unit cell parameters a= b= 229.10, c= 63.49 Å. There are 2 or 3 molecules in the asymmetric unit.